ABSTRACT
Objective:To search for the efficient immune adjuvant for rotavirus DNA vaccine by evaluating the efficacy in modifying immune responses to rotavirus.Methods:BALB/c mice were immunized by intramuscular and intranasal routes with 3 doses of naked DNA and liposome-coated DNA vaccine at two-week intervals. Titers of serum IgG and IgA were measured by ELISA.Results:Serum titers of rotavirus specific IgG in naked DNA and liposome-coated DNA vaccine-immunized mice were significantly higher than that of the unimmunized controls. Interestingly, only those mice administrated with liposome-coated DNA vaccine by intramuscular route generated serum rotavirus IgA,and neither by naked plasmid nor by intranasal immunization with DNA-liposome complex.Conclusion:These results indicate that liposome can act as both the vector and adjuvant for DNA vaccine against rotavirus by intramuscular injection,which induces rotavirus-specific IgA in the sera,and may provide partial protection against rotavirus infection.